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However, mass spectrometry, while advantageous for analyzing whole cell association, has principal limitations as currently reported: (a) restricted throughput capacity and (b) would ambiguously define molecule location unless additional, careful fractionation methods are included to show periplasmic or cytoplasmic accumulation 18 , and (c) needs compounds to be mass active

e.g., other belladonna alkaloids, antihistamines (including meclizine), tricyclic antidepressants, and muscle relaxants